1.
Sources and Composition
1.1
Sources
Emblica officinalis (of the family Euphorbiaceae), also known as Amla or Amla churna (the latter referring to the dried fruits) and is a component of some Ayurveda combination therapies such as Chyawanprash[1] and Triphala.[2] This plant is synonymous with Phyllanthus emblica and Amla is sometimes used interchangeably with Anwala, Amlaj, or Amlaki.[3][4] It is also known as Indian gooseberry.[5]
The fruits are traditionally used in Egypt for improving memory, as a stimulant, and restoratives for all organs[6][7] while in general it is used for the treatment or prevention of diarrhea, jaundice, inflammation, cerebral insufficiency and mental disorders[8] while other claims include usage as an appetite stimulant or for the treatment of diabetes.[9] Among some ayurvedic texts (Charaka Samhita and Sushruta Samhita), this herb is referred to as the "best among the sour fruits" or "the best among rejuvenative herbs".[4]
The fruits appear to also be well known in regards to their taste, which is described as being a relative balance of five major senses (sweet, sour, pungent, bitter, and astringent).[9]
Emblica officinialis (Amla) is a fruit-bearing plant that has traditionally been used for whole-body medicinal purposes, and appears to be fairly well acclaimed in traditional medicine. The dried fruit powder is referred to as Amla Churna and is the commonly supplemented part
1.2
Composition
When consuming the fruits themselves, emblica officinalis contributes:
- 77+/-3kcal per 100g (assuming a 80.3+/-0.8% moisture content)[10]
- 0.5+/-0.1g dietary protein per 100g[10]
- 18.7+/-0.8g dietary carbohydrate per 100g[10] some of which are conjugated to phenolics[11]
- Trace fatty acids[10]
- 6.1+/-0.6g dietary fiber per 100g[10]
Similar to most fruits, Amla fruits are mostly carbohydrate with a decent fiber content and next to no protein nor fatty acids
The components of emblica officinalis include (fruit unless otherwise specified):
- Phyllemblin[9]
- Emblicanin A and B (major tannoids)[9] which can be hydrolyzed to form gallic acid and ellagic acid with glucose (Emblicanin A) or ellagic acid with glucose (Emblicanin B)
- Punigluconin and pedunculagin[9]
- Putranjivain A[12]
- Progallin A (leaves)[13]
- Tannin structures elaeocarpusin and chebulagic acid (also found in Dimocarpus Longan)[12] and various configuations of these tannic acids with gallic acid[12]
- β-glucogallin (1-O-galloyl-β-d-glucose)[14]
- Gallic Acid[15]
- Mucic acid 3-O-gallate[16][12]
- Quercetin at 2.6+/-1.6mg/100g (no detectable kaempferol, Myricetin, chlorogenic acid, ferulic acid, nor apigenin)[10]
- Phytosterols including β-sitosterol (12.12+/-1.90mg/100g), stigmasterol (0.70+/-0.21mg/100g), and campesterol (0.26+/-0.02mg/100g)[17] as well as trihydroxysitosterol and 5α,6β,7α-7α-acetoxysitosterol[18]
- Carotenoids including lutein (49+/-27mg/100g) and β-carotene (32+/-19mg/100g)[10]
- Vitamin C (2% of fruit juice[17] or 575+/-452mg/100g of the fruits (wet weight)[10])
- Vitamin E at 0.16 ± 0.05mg/100g fruit wet weight[10]
- Calcium (42+/-12mg/100g), Sodium (13+/-4mg/100g), Phosphorus (21+/-5mg/100g), Potassium (151+/-37mg/100g or 1.16+/-0.05%), Magnesium (13+/-2mg/100g), Manganese (0.71+/-0.06mg/g) Iron (0.16+/-0.04mg/100g), Copper (0.04+/-0.03mg/100g), chloride (25.6+/-2.3mg/g) and Zinc (0.14+/-0.08mg/100g)[10][19] with the content varying depending on analysis technique
Appears to be particularly rich in vitamin C, with some other unique bioactives. Most of the bioactives seem to belong to the class of tannin structures, which are large antioxidant structures particularly high in a few select plants or fruits
The total phenolic content of the fruits appears to be 3703+/-1244mGAE/100g (Gallic acid equivalents)[10] and the total tannoid content (emblicanins, Punigluconin, gallic acid, rutin, and pedunculagin) consist of approximately 10-13% of the fruit by dry weight.[20][9]
Despite the fruits having no detectable fatty acids, the seeds possess an oil that is rich in linoleic acid (44.0%) and oleic acid (28.4%).[9]
1.3
Physicochemical Properties
The antioxidant potential of the herb does not seem to be altered with storage at 4°C for one years time.[21]
1.4
Variants and Formulations
There is an ayuvedic and mid-eastern combination formula known as Triphala or Itrifal Saghir which conists of equal parts of the seedless fruits from emblica officinalis, terminalia belerica, and terminalia chebula and appears to be taken at 10g overall (5g twice daily) after being mixed with almond oil and kneaded with frothless honey.[22]
2.
Longevity
2.1
Non-Mammalian Interventions
Emblica offinicalis appears to be of interest for longevity as, according to the Acharya Charaka (Ayurveda test), the fruits have been reported to be the best to increase longevity.[23]
A study in drosophilia noted that daily consumption of feed with an Amla content was able to increase the days survived from 41.9 days to 76.8 days (83% increase), although a large variance was detected (76.8 days being the mean value, with +/-40.6 days for maximal and minimal values).[23] This increase in longevity was met with an increase in fecundity.[23]
Limited evidence to support the longevity promoting effects of Amla, but said preliminary evidence suggests surprisingly potent effects
3.
Neurology
3.1
Mechanisms
Oral ingestion of 200mg/kg emblica officinalis daily over 14 days has been found to reduce concentrations of MAO-A in the brains of mice, and this was not accompanied by any alterations in locomotion.[24]
Has been noted to reduce MAOA activity, which may cause an increase in neurotransmitters (catecholamines and serotonin implicated)
3.2
Cholinergic Neurotransmission
Emblica officinalis does not appear to interact with the acetylcholinesterase enzyme at concentrations up to 1mg/mL in vitro[7] although the increase in this enzyme seen with anticholinergic treatment can be reduced with 600mg/kg of the herb (comparable potency to 200mg/kg Piracetam)[25] or the increase seen with aging reduced by 13.5-23.7% with 50-200mg/kg oral ingestion of the fruit (although there was a 10.9-22.8% reduction in young mice as well).[4]
Although it does not appear to directly inhibit the enzyme, but increases in the acetylcholine enzyme that may occur (either during aging or anticholinergic treatment) might be attenuated with the plant
3.3
Neuroprotection
Emblica officinalis appears scavenge free radicals with an IC50 of 6.3µg/mL (DPPH), although this is less potent than both Terminalia Arjuna (3.1µg/mL) and chebula (2.2µg/mL) as well as the reference quercetin (4.5µg/mL)[7] while being more potent than Anacyclus Pyrethrum (26.3µg/mL).[7] Similar potencies have been noted elsewhere,[26] suggesting that while Amla is potent there are plants and compounds with higher efficacy.
Thought to be secondary to these antioxidative effects, oral ingestion of 250mg/kg daily for six weeks alongside chronic alcohol ingestion is able to confer protective effects against mitochondrial dysfunction[27] and pretreatment of 500-700mg/kg of the fruit extract before kainate administration (convulsant that induces seizures associated with prooxidative changes[28]) can reduce the subsequent seizures and cogntive impairment.[29] Similar protective effects are seen against PTZ-induced seizures.[30]
Emblica officinalis appears to exert general antioxidative effects in brain cells, which are thought to underlie some of the protective effects. This has been confirmed with oral ingestion of the fruits in the standard dosages in rodents
It does not have any influence on COX1 activity up to 1mg/mL.[7]
3.4
Memory and Learning
50-200mg/kg of oral fruit extract over 15 days to both young and old rats was able to dose-depedently reduce transfer and step-down latency in an elevated maze plus test in both age groups[4] with 400mg/kg Piracetam being comparable potency to 200mg/kg Amla.[4] This study is duplicated in Medline.[3]
Animal evidence supports the usage of Amla as a nootropic in otherwise healthy and young animals, and the benefits also appear to extend to older animals. The nootropic potential seems similar to Piracetam
In rats given a hydroalcoholic extract of emblica officinalis (150-600mg/kg) for seven days intraperitoneally prior to scopolamine injections, supplementation was able to normalize the toxin-induced oxidative and learning changes with 600mg/kg being of comparable potency to 200mg/kg Piracetam.[25] Elsewhere, the amnesiac effects of scopolamine or diazepam have been noted to be prevented with 200mg/kg oral Amla (comparable to 400mg/kg Piracetam).[4]
Appears to have anti-amnesiac effects, of somewhat comparable potency to Piracetam as the reference drug
3.5
Depression and Anxiety
In isolation, Amla at 200-400mg/kg oral ingestion to mice for 14 days noted that the lower dose was effective in exerting antidepressant effects in forced swim and tail suspension tests (400mg/kg less effective) with a potency comparable to the reference drugs (15mg/kg imipramine, 20mg/kg phenelzine, and 20mg/kg fluoxetine).[24] This is thought to be due to the suppressive effects of Amla on MAOA,[24] an enzyme that degrades monoamines (catecholamines and serotonin, as well as GABA).
It has also shown anti-anxiety (anxiolytic) properties at 0.8-4mg/kg bodyweight in rats as assessed by elevated maze testing.[31]
The compound supplement Rasayana Ghana (Emblica officinalis fruits with tribulus terrestris and Tinospora cordifolia in equal parts) at 260mg/kg appears to confer antidepressant effects as well as anxiolytic, although its potency is less than diazepam (2mg/kg for anxiolysis) and imipramine (5mg/kg for depression).[32]
A combination supplement has noted weak anti-depressive and anxiolytic effects, and no evidence exists on Amla used in isolation
4.
Cardiovascular Health
4.1
Cardiac Tissue
Gallic acid (one of the tannin structures in emblica officinalis[33]) at 25-100mg/kg oral ingestion has been noted to have cardioprotective effects against the state of diabetes in rats in a dose-dependent manner[34] and this has been noted with the juice of the fruits.[35] This is thought to be due to the antioxidative effects of the plant, as cardioprotection has been noted in instances where blood glucose is not reduced.[35]
Amla (100-500mg/kg over 30 days) has also shown cardioprotective effects against isoproterenol toxicity.[36]
4.2
Endothelium
20-100µg/mL of the fruit extract appears to be protective against H2O2 induced endothelial cell death, associated with Akt activation without NF-kB[37] and a similar concentration (1-100µg/mL) has been noted to inhibit LPS-induced coagulation.[38]
Emblica officinalis has shown wound healing effects in isolated HUVEC cells at 0.1μg/mL over 48 hours of incubation, although this was also seen with isolated vitamin c at 0.01μg/mL, and 1μg/mL was able to promote endothelial cell migration with a potency comparable to 100ng/mL VEGF and possibly working vicariously through VEGF (as its mRNA was increased).[21]
Emblica officinalis at 10μg/mL appears to enhance nitric oxide formation in isolated HUVECs to around 140% of control, with lower doses being ineffective.[21] This was not related to the vitamin C content (not replicated by 1μg/mL vitamin C)[21] and was noted in vivo when 75-300mg/kg of the fruits to rats over five weeks increased serum nitric oxide and eNOS activity in cardiac tissue.[39]
4.3
Cholesterol and Triglycerides
The oxidation of LDL has been noted to be reduced with emblica officinalis fruits in vitro quite weakly, with an IC50 of 136.3μg/mL.[40]
Can be forced to inhibit LDL oxidation, but seems to occur at rather high concentrations and may not be biologically relevant
Oral administration of 50-200mg/kg Amla has been noted to reduce cholesterol in both young (13.6-27.5%) and old (7.6-23.4%) rats with the 100-200mg/kg dosage range over 15 days being comparable to the reference drug simvastatin (5mg/kg).[4] Lower dose amla (10-40mg/kg) also seems effective over 100 days in rats, although to a lesser degree.[17]
Serum triglycerides have been noted to be reduced in rats with 10mg/kg of the ethyl acetate fraction or 40mg/kg of fruit juice.[17]
In rats given the fruits or leaves, there appear to be reductions in both cholesterol and triglycerides
Reductions have been noted in total cholesterol and LDL-C, as well as triglycerides, with oral intake of 3g of the fruits of emblica officinalis daily for 21 days; an increase in HDL-C was also seen at this dose, and it affected both healthy controls and the diabetics under investigation.[41]
Benefits appear to extend to humans
5.
Interactions with Glucose Metabolism
5.1
Mechanisms
Emblica officinalis fruits have been found to inhibit enzymes of carbohydrate absorption, including α-glucosidase (IC50 of 1.0µg/mL) and α-amylase (IC50 of 94.3µg/mL).[40]
May be able to inhibit carbohydrate absorption enzymes, with the potency against α-glucosidase being fairly respectable
Glycation (a reduction between sugars and amino acids) has been noted to be reduced with the fruits, albeit with an IC50 of 182.9µg/mL.[40]
May reduce glycation at the concentrations that it also reduces LDL oxidation (ie. high enough that it might not be biologically relevant) which is thought to simply be due to antioxidative effects
A molecule in emblica officinalis known as β-glucogallin (1-O-galloyl-β-d-glucose) appears to inhibit the human aldose reductase enzyme with an IC50 of 17µM[42] which is thought to be biologically relevant since rodent interventions have noted protective effects (reduced oxidation and cataract formation) with a similar phenotype to aldose reductase inhibition.[43][20]
May be inhibitory against aldose reductase
5.2
Blood Glucose
In diabetic rats given the hydromethanolic (20:80) extract of the leaves of emblica officinalis (100-400mg/kg bodyweight for 45 days) noted dose-dependent reductions in blood glucose and increases in serum insulin, with all groups being unable to outperform the reference drug (1mg/kg glibenclamide).[44]
Reductions in plasma glucose have also been noted in a model of fluoride toxicity (known to have the side-effect of hyperglycemia[45]) when 2.5-10% of the diet was comprised of amla fruits.[46]
Rat studies with abnormally elevated blood glucose noted reductions with oral ingestion of both the leaves and fruits of emblica officinalis
Amla's effects on blood glucose in humans has been reviewed.[47] It has been used in a combination supplement (alongside Bitter Gourd, Gurmur Leaves, Jamun Fruit and Holy basil) to reduce blood glucose in type II diabetics[48] and elsewhere in type II diabetics with other herbs (Salacia oblonga and curcuma longa).[49] Only one trial has been conducted using Amla in isolation (noted in the review,[47] unable to be located online) where a 35g fresh amla fruit was consumed daily for two months noted that while there was no overall reduction in blood glucose that those with a baseline glucose over 150mg/dL experienced a statistically significant reduction.
Another study in uremic patients with 100mg emblica officinalis paired with 100mg EGCG (one of the green tea catechins) noted a reduction in fasting blood glucose without any influence on HbA1c, but is again confounded.[50]
In diabetic and healthy humans given 1-3g of powdered emblica fruits daily for 21 days has noted reduction in postprandial glucose (highest dose being the only effect one in healthy controls, 3g being as effective as glibenclamide at 5mg twice daily) and fasting glucose (all groups and doses, with 3g being as effective as glibenclamide).[41]
The majority of research on Emblica officinalis fruits has been confounded with the inclusion of other herbs that may also have hypoglycemic effects. Despite this lacklustre evidence, the lone study using a blinded protocol noted potency comparable to glibenclamide
5.3
Insulin Sensitivity
Emblica officinalis has shown insulin sensitizing properties in adipocytes by increasing glucose uptake in the 50-200µg/mL range (dose-dependent)
6.
Fat Mass and Obesity
6.1
Mechanisms
200μg/mL of the fruit extract (hydroalcoholic extract) appears to increase glucose uptake into adipocytes, and while comparable in potency to insulin it was antagonistic with incubated insulin.[51]
6.2
Interventions
Administration of amla (40mg/kg juice or 10mg/kg ethanolic extract) for 100 days to rats has failed to significantly reduce food intake or body weight.[17]
In humans, Triphala (Amla with the two species of terminalia known as chebula and bellerica in equal ratios) at 5g twice daily over the course of three months in obese individuals without modifications to the diet (although diet was monitored to ensure it did not change significantly) noted that supplementation was associated with a 4.5% reduction in weight (4.37kg) and 3cm reduction in waist circumference while placebo did not alter their weight.[22]
7.
Interactions with Hormones
7.1
Testosterone
Emblica officinalis has been tested for its 5α-reductase inhibiting properties, and appears to be fairly potent as 1g of the crude fruit extract was as potent as 18.99+/-0.40mg finasteride.[52] It was less potent than Carthamus tinctorius (1g being equivalent to 24.30+/-1.64mg finasteride), and these inhibitory effects were thought to underlie the hair growth promoting effects of topical application.[52]
Emblica officinalis appears to be a fairly respectable 5α-reductase inhibitor in preliminary testing
8.
Inflammation and Immunology
8.1
Mechanisms
The increase in NF-kB expression in the liver of rats during the aging process is attenuated with oral intake of 10-40mg/kg of emblica officinalis.[17]
9.
Interactions with Oxidation
9.1
Interventions
10-40mg/kg of the fruit daily for 100 days in rats has been noted to reduce serum TBARS concentrations (22-36%)[17]
10.
Interactions with Organ Systems
10.1
Eyes
β-glucogallin (1-O-galloyl-β-d-glucose) is an aldose reductase inhibitor with an IC50 of 17µM and is able to inhibit sorbitol accumulation by 73% at 30µM.[42] Aldose reductase is the first and rate-limiting enzyme of polyol synthesis[53] and is upregulated in diabetes[54][55] and contributes to pathology, thus its inhibition is desired for controlled symptoms of diabetes beyond blood glucose such as diabetic retinopathy or nephropathy.[56]
LPS-induced inflammation can be inhibited by aldose reductase inhibitors (as seen with established ARIs[57][58]) which extends to β-glucogallin as evidenced by a 10mg/kg injections of this molecule with equal potency as the reference drug (sorbinil).[14]
The whole plant extract of emblica officinalis (either at 2% of the diet or 0.2% of isolated tannoid structures) is able to attenuate the rate of cataracht formation in diabetic rats and reduce buildup of sorbitol in the eyes, but without apparent affect on blood glucose or insulin.[20]
Tannoids from emblica officinalis appear to inhibit aldose reductase and may reduce the progression of some diabetes comorbidities without affecting blood glucose or insulin, this includes the formation of cataracts and diabetic retinopathy
10.2
Liver
Liver cholesterol and triglyceride content have been noted to be reduced with 10mg/kg of the ethyl acetate fraction of amla or 40mg/kg of the fruit over 100 days in rats.[17]
Oral feeding of 5-10% of the diet as Amla churna (dried fruit powder) to rats subsequently given the pro-oxidant hexachlorocyclohexane appeared to exert some protective effects via reducing oxidative damage.[8]
11.
Interactions with Cancer Metabolism
11.1
Cervical Cancer
The fruit pulp of emblica officinalis (water extract) at 10-300μg/mL in HeLa and SiHa cells (two cervical cancer cell lines) showed concentration dependent inhibition of AP-1 binding to DNA with up to 90% inhibition at the highest tested dose of 300μg/mL.[5] It took up to 12 hours to manifest (suggestive of protein induction), and it was later found that cervical cancer cells that tested positive for HPV (which is known to activate AP-1 signalling[59][60]) were sensitive to emblica officinalis since it could inhibit viral replication at concentrations as low as 30μg/mL.[5]
Regardless of HPV infections, emblica officinalis appears to induce apoptosis in cervical cancer cells with a decrease in cells in the G0/G1 phase and an increase in sub-G1 phase.[5]
Mostly appears to be anti-proliferative in cervical cancer cells that express HPV (Human papillomavirus)
12.
Interactions with Aesthetics
12.1
Hair
Emblica officinalis appears to be recommended as a Thai rememdy for hair regrowth (fried in sesame seed oil, then topically applied), and topical application of 1% of the fruit extract in solution to mice was able to increase hair follicle count (91.7%) to a greater degree than 2% minoxidil (50%) but less than Clitoria Ternatea (117%) and Carthamus tinctorius (187%).[52]
Appears to promote hair growth when topically applied, and its potency is greater than the reference drug of Rogaine
12.2
Skin
Secondary to its antioxidant properties emblica officinalis appears to be capable of protecting skin cells from UV radiation by reducing the increase in reactive oxygen species from 84+/-1.4% (control cells) down to 15+/-4% (at 0.5mg/mL), which outperformed pure vitamin c at the same concentration (64+/-2%).[61] This protective effect has been noted at concentrations as low as 10-40μg/mL.[62]
Appears to exert protective effects against oxidation in skin cells, and appears to be fairly potent at doing so
13.
Nutrient-Nutrient Interactions
13.1
Triphala
Triphala is a combination of three herbs, one of which includes emblica officinalis whereas the other two herbs come from the genus Terminalia (chebula and bellirica).[63]
14.
Safety and Toxicology
14.1
General
In rats, oral adminsitration of the hydromethanolic (20:80) extract up to 2,000mg/kg over 15 days has failed to cause any clinical signs of toxicity.[44]
References
- ^Manjunatha S, Jaryal AK, Bijlani RL, Sachdeva U, Gupta SKEffect of Chyawanprash and vitamin C on glucose tolerance and lipoprotein profileIndian J Physiol Pharmacol.(2001 Jan)
- ^Baliga MSTriphala, Ayurvedic formulation for treating and preventing cancer: a reviewJ Altern Complement Med.(2010 Dec)
- ^Vasudevan M, Parle MEffect of Anwala churna (Emblica officinalis GAERTN.): an ayurvedic preparation on memory deficit ratsYakugaku Zasshi.(2007 Oct)
- ^Vasudevan M, Parle MMemory enhancing activity of Anwala churna (Emblica officinalis Gaertn.): an Ayurvedic preparationPhysiol Behav.(2007 May 16)
- ^Mahata S, Pandey A, Shukla S, Tyagi A, Husain SA, Das BC, Bharti ACAnticancer Activity of Phyllanthus emblica Linn. (Indian Gooseberry): Inhibition of Transcription Factor AP-1 and HPV Gene Expression in Cervical Cancer CellsNutr Cancer.(2013)
- ^Herbal medicine in ancient Egypt
- ^Ali SK, Hamed AR, Soltan MM, Hegazy UM, Elgorashi EE, El-Garf IA, Hussein AAIn-vitro evaluation of selected Egyptian traditional herbal medicines for treatment of alzheimer diseaseBMC Complement Altern Med.(2013 May 30)
- ^Anilakumar KR, Nagaraj NS, Santhanam KReduction of hexachlorocyclohexane-induced oxidative stress and cytotoxicity in rat liver by Emblica officinalis gaertnIndian J Exp Biol.(2007 May)
- ^EVALUATION OF PHYSICOCHEMICAL AND PRELIMINARY PHYTOCHEMICAL STUDIES ON THE FRUIT OF EMBLICA OFFICINALIS GAERTN
- ^Judprasong K, Charoenkiatkul S, Thiyajai P, Sukprasansap MNutrients and bioactive compounds of Thai indigenous fruitsFood Chem.(2013 Oct 1)
- ^Chatterjee UR, Bandyopadhyay SS, Ghosh D, Ghosal PK, Ray BIn vitro anti-oxidant activity, fluorescence quenching study and structural features of carbohydrate polymers from Phyllanthus emblicaInt J Biol Macromol.(2011 Nov 1)
- ^Yang B, Kortesniemi M, Liu P, Karonen M, Salminen JPAnalysis of hydrolyzable tannins and other phenolic compounds in emblic leafflower (Phyllanthus emblica L.) fruits by high performance liquid chromatography-electrospray ionization mass spectrometryJ Agric Food Chem.(2012 Sep 5)
- ^Zhong ZG, Wu DP, Huang JL, Liang H, Pan ZH, Zhang WY, Lu HMProgallin A isolated from the acetic ether part of the leaves of Phyllanthus emblica L. induces apoptosis of human hepatocellular carcinoma BEL-7404 cells by up-regulation of Bax expression and down-regulation of Bcl-2 expressionJ Ethnopharmacol.(2011 Jan 27)
- ^Chang KC, Laffin B, Ponder J, Enzsöly A, Németh J, LaBarbera DV, Petrash JMBeta-glucogallin reduces the expression of lipopolysaccharide-induced inflammatory markers by inhibition of aldose reductase in murine macrophages and ocular tissuesChem Biol Interact.(2013 Feb 25)
- ^Amaravani M, Prasad NK, Ramakrishna VCOX-2 structural analysis and docking studies with gallic acid structural analoguesSpringerplus.(2012 Dec)
- ^She G, Cheng R, Sha L, Xu Y, Shi R, Zhang L, Guo YA novel phenolic compound from Phyllanthus emblicaNat Prod Commun.(2013 Apr)
- ^Yokozawa T, Kim HY, Kim HJ, Okubo T, Chu DC, Juneja LRAmla (Emblica officinalis Gaertn.) prevents dyslipidaemia and oxidative stress in the ageing processBr J Nutr.(2007 Jun)
- ^Qi WY, Li Y, Hua L, Wang K, Gao KCytotoxicity and structure activity relationships of phytosterol from Phyllanthus emblicaFitoterapia.(2013 Jan)
- ^Waheed S, Fatima IInstrumental neutron activation analysis of Emblica officinalis, Terminalia belerica and Terminalia chebula for trace element efficacy and safetyAppl Radiat Isot.(2013 Jul)
- ^Suryanarayana P, Saraswat M, Petrash JM, Reddy GBEmblica officinalis and its enriched tannoids delay streptozotocin-induced diabetic cataract in ratsMol Vis.(2007 Jul 24)
- ^Chularojmontri L, Suwatronnakorn M, Wattanapitayakul SKPhyllanthus emblica L. Enhances Human Umbilical Vein Endothelial Wound Healing and SproutingEvid Based Complement Alternat Med.(2013)
- ^Kamali SH, Khalaj AR, Hasani-Ranjbar S, Esfehani MM, Kamalinejad M, Soheil O, Kamali SAEfficacy of 'Itrifal Saghir', a combination of three medicinal plants in the treatment of obesity; A randomized controlled trialDaru.(2012 Sep 10)
- ^Pathak P, Prasad BR, Murthy NA, Hegde SNThe effect of Emblica officinalis diet on lifespan, sexual behavior, and fitness characters in Drosophila melanogasterAyu.(2011 Apr)
- ^Dhingra D, Joshi P, Gupta A, Chhillar RPossible involvement of monoaminergic neurotransmission in antidepressant-like activity of Emblica officinalis fruits in miceCNS Neurosci Ther.(2012 May)
- ^Golechha M, Bhatia J, Arya DSStudies on effects of Emblica officinalis (Amla) on oxidative stress and cholinergic function in scopolamine induced amnesia in miceJ Environ Biol.(2012 Jan)
- ^Sarkar R, Mandal NHydroalcoholic extracts of Indian medicinal plants can help in amelioration from oxidative stress through antioxidant propertiesJ Complement Integr Med.(2012)
- ^Reddy VD, Padmavathi P, Kavitha G, Gopi S, Varadacharyulu NEmblica officinalis ameliorates alcohol-induced brain mitochondrial dysfunction in ratsJ Med Food.(2011 Jan-Feb)
- ^Liang LP, Patel MSeizure-induced changes in mitochondrial redox statusFree Radic Biol Med.(2006 Jan 15)
- ^Golechha M, Bhatia J, Ojha S, Arya DSHydroalcoholic extract of Emblica officinalis protects against kainic acid-induced status epilepticus in rats: evidence for an antioxidant, anti-inflammatory, and neuroprotective interventionPharm Biol.(2011 Nov)
- ^Golechha M, Bhatia J, Arya DSHydroalcoholic extract of Emblica officinalis Gaertn. affords protection against PTZ-induced seizures, oxidative stress and cognitive impairment in ratsIndian J Exp Biol.(2010 May)
- ^Antianxiety effect of aqueous extract of fruits of Emblica officinalis on acute and chronic administration in rats
- ^Deole YS, Chavan SS, Ashok BK, Ravishankar B, Thakar AB, Chandola HMEvaluation of anti-depressant and anxiolytic activity of Rasayana Ghana Tablet (A compound Ayurvedic formulation) in albino miceAyu.(2011 Jul)
- ^Bhattacharya A, Chatterjee A, Ghosal S, Bhattacharya SKAntioxidant activity of active tannoid principles of Emblica officinalis (amla)Indian J Exp Biol.(1999 Jul)
- ^Patel SS, Goyal RKCardioprotective effects of gallic acid in diabetes-induced myocardial dysfunction in ratsPharmacognosy Res.(2011 Oct)
- ^Patel SS, Goyal RKPrevention of diabetes-induced myocardial dysfunction in rats using the juice of the Emblica officinalis fruitExp Clin Cardiol.(2011 Fall)
- ^Ojha S, Golechha M, Kumari S, Arya DSProtective effect of Emblica officinalis (amla) on isoproterenol-induced cardiotoxicity in ratsToxicol Ind Health.(2012 Jun)
- ^Wongpradabchai S, Chularojmontri L, Phornchirasilp S, Wattanapitayakul SKProtective effect of Phyllanthus emblica fruit extract against hydrogen peroxide-induced endothelial cell deathJ Med Assoc Thai.(2013 Jan)
- ^Pradyumna Rao T, Okamoto T, Akita N, Hayashi T, Kato-Yasuda N, Suzuki KAmla (Emblica officinalis Gaertn.) extract inhibits lipopolysaccharide-induced procoagulant and pro-inflammatory factors in cultured vascular endothelial cellsBr J Nutr.(2013 Jun 7:1-6)
- ^Bhatia J, Tabassum F, Sharma AK, Bharti S, Golechha M, Joshi S, Sayeed Akhatar M, Srivastava AK, Arya DSEmblica officinalis exerts antihypertensive effect in a rat model of DOCA-salt-induced hypertension: role of (p) eNOS, NO and oxidative stressCardiovasc Toxicol.(2011 Sep)
- ^Nampoothiri SV, Prathapan A, Cherian OL, Raghu KG, Venugopalan VV, Sundaresan AIn vitro antioxidant and inhibitory potential of Terminalia bellerica and Emblica officinalis fruits against LDL oxidation and key enzymes linked to type 2 diabetesFood Chem Toxicol.(2011 Jan)
- ^Akhtar MS, Ramzan A, Ali A, Ahmad MEffect of Amla fruit (Emblica officinalis Gaertn.) on blood glucose and lipid profile of normal subjects and type 2 diabetic patientsInt J Food Sci Nutr.(2011 Sep)
- ^Puppala M, Ponder J, Suryanarayana P, Reddy GB, Petrash JM, LaBarbera DVThe isolation and characterization of β-glucogallin as a novel aldose reductase inhibitor from Emblica officinalisPLoS One.(2012)
- ^Suryanarayana P, Kumar PA, Saraswat M, Petrash JM, Reddy GBInhibition of aldose reductase by tannoid principles of Emblica officinalis: implications for the prevention of sugar cataractMol Vis.(2004 Mar 12)
- ^Nain P, Saini V, Sharma S, Nain JAntidiabetic and antioxidant potential of Emblica officinalis Gaertn. leaves extract in streptozotocin-induced type-2 diabetes mellitus (T2DM) ratsJ Ethnopharmacol.(2012 Jun 26)
- ^Chlubek D, Grucka-Mamczar E, Birkner E, Polaniak R, Stawiarska-Pieta B, Duliban HActivity of pancreatic antioxidative enzymes and malondialdehyde concentrations in rats with hyperglycemia caused by fluoride intoxicationJ Trace Elem Med Biol.(2003)
- ^Vasant RA, Narasimhacharya AVAmla as an antihyperglycemic and hepato-renal protective agent in fluoride induced toxicityJ Pharm Bioallied Sci.(2012 Jul)
- ^Deng RA review of the hypoglycemic effects of five commonly used herbal food supplementsRecent Pat Food Nutr Agric.(2012 Apr 1)
- ^Effects of a Composite of Tulsi Leaves, Amla, Bitter Gourd, Gurmur Leaves, Jamun Fruit and Seed in Type 2 Diabetic Patients
- ^Faizal P, Suresh S, Satheesh Kumar R, Augusti KTA study on the hypoglycemic and hypolipidemic effects of an ayurvedic drug Rajanyamalakadi in diabetic patientsIndian J Clin Biochem.(2009 Jan)
- ^Chen TS, Liou SY, Wu HC, Tsai FJ, Tsai CH, Huang CY, Chang YLEfficacy of epigallocatechin-3-gallate and Amla (Emblica officinalis) extract for the treatment of diabetic-uremic patientsJ Med Food.(2011 Jul-Aug)
- ^Kalekar SA, Munshi RP, Bhalerao SS, Thatte UMInsulin sensitizing effect of 3 Indian medicinal plants: an in vitro studyIndian J Pharmacol.(2013 Jan-Feb)
- ^Kumar N, Rungseevijitprapa W, Narkkhong NA, Suttajit M, Chaiyasut C5α-reductase inhibition and hair growth promotion of some Thai plants traditionally used for hair treatmentJ Ethnopharmacol.(2012 Feb 15)
- ^Bhatnagar A, Srivastava SKAldose reductase: congenial and injurious profiles of an enigmatic enzymeBiochem Med Metab Biol.(1992 Oct)
- ^Gabbay KHThe sorbitol pathway and the complications of diabetesN Engl J Med.(1973 Apr 19)
- ^Ghahary A, Luo JM, Gong YW, Chakrabarti S, Sima AA, Murphy LJIncreased renal aldose reductase activity, immunoreactivity, and mRNA in streptozocin-induced diabetic ratsDiabetes.(1989 Aug)
- ^Tomlinson DRAldose reductase inhibitors and the complications of diabetes mellitusDiabet Med.(1993 Apr)
- ^Ramana KV, Reddy AB, Tammali R, Srivastava SKAldose reductase mediates endotoxin-induced production of nitric oxide and cytotoxicity in murine macrophagesFree Radic Biol Med.(2007 Apr 15)
- ^Ramana KV, Fadl AA, Tammali R, Reddy AB, Chopra AK, Srivastava SKAldose reductase mediates the lipopolysaccharide-induced release of inflammatory mediators in RAW264.7 murine macrophagesJ Biol Chem.(2006 Nov 3)
- ^Soto U, Das BC, Lengert M, Finzer P, zur Hausen H, Rösl FConversion of HPV 18 positive non-tumorigenic HeLa-fibroblast hybrids to invasive growth involves loss of TNF-alpha mediated repression of viral transcription and modification of the AP-1 transcription complexOncogene.(1999 May 27)
- ^Thierry F, Spyrou G, Yaniv M, Howley PTwo AP1 sites binding JunB are essential for human papillomavirus type 18 transcription in keratinocytesJ Virol.(1992 Jun)
- ^Majeed M, Bhat B, Anand S, Sivakumar A, Paliwal P, Geetha KGInhibition of UV-induced ROS and collagen damage by Phyllanthus emblica extract in normal human dermal fibroblastsJ Cosmet Sci.(2011 Jan-Feb)
- ^Adil MD, Kaiser P, Satti NK, Zargar AM, Vishwakarma RA, Tasduq SAEffect of Emblica officinalis (fruit) against UVB-induced photo-aging in human skin fibroblastsJ Ethnopharmacol.(2010 Oct 28)
- ^Baliga MS, Meera S, Mathai B, Rai MP, Pawar V, Palatty PLScientific validation of the ethnomedicinal properties of the Ayurvedic drug Triphala: a reviewChin J Integr Med.(2012 Dec)