1.
Sources and Composition
Apium graveolens (of the family Apiaceae) is a vegetable commonly referred to as 'Celery', which has apparently been cultivated for over 3000 years mostly in Egypt.[1] The term 'celeriac' refers to a variant of this species known as apium graveolens var. rapaceum.[2] Despite the stem being the most commonly ingested portion of the plant as a common vegetable, the seeds of celery appears to have been used for medicinal purposes (Egypt and China) to treat; bronchitis, asthma, liver and spleen diseases and with hepatoprotective activity against many hepatotoxins.[3] It has also been reported to be diluted in beverages and drunk with wine to cool a hot temper.[4]
The aroma and characteristic taste of celery is thought to be secondary to the sedanenolide component.[5][6]
The difference between the 'vegetable' or 'stem' (edible portion of celery commonly seen in grocery stores) and the seeds or seed oil (common component of supplement) should be emphazied. The information below may not apply to vegetable consumption if using the seed oil
1.1
Composition
Celery seed oil is comprised of both the oil component (fatty acids) and the volatile component (small molecular weight molecules); the fatty acids in celery seed oil include:
Whereas the volatile component of celery (between 0.25%[7] and 1.5-3.0%[5][8] of celery by weight) tends to contain:
- Sedanolide (aka. 3-butyltetrahydrophthalide; 24%[8])
- 3-butylphthalide (22%[8])
- 4,7-dimethoxy-5-(prop-2-enyl)benzo-1,3-dioxolan or apiole (23%[8])
- Ligustilide[9] (also present in Angelica sinensis,[10] and Ligusticum chuanxiong,[11])
- Neophytadiene (34.6–36.7% of the extract in italian sourced volatiles, 0.5-5.2% in Portuguese[12]) and neocnidilide (36.2–45.2% in Portuguese extracts, 7.6-19.5% in italian[12])
- Other minor phthalide compounds such as p-heptylphenol[13] methyl sedanonate (trace),[2] cis-sedanolide (5.0%) and trans-sedanolide (1.4%) cnidilide.[2]
- β-pinene (19.4%) and α-pinene (1.3%)[7]
- Camphene (0.3%)[7]
- Limonene (35.5-39.4%,[7][2] some sources note a variability as low as 5.6%[12])
- α-thuyene[7]
- β-phellendrene (3.6%)[7]
- p-cymene (0.4%)[7]
- γ-terpinene (12.5%)[7]
- Sabinene (1.5%)[7]
- β-Selinene (3.2%)[7]
- γ-Dodecalactone (0.7%)[7]
- γ-himachalene (10.3% in portuguese volatiles, 0.3% in italian[12])
- Terpinolene (0.3%)[7]
- Linalool (6.2%)[7]
- Nonanal (1.7%)[7]
For the most part, the volatile oil of celery is comprised of limonene and a large amount of monoterpenoid volatiles and a large phthalide content. The phthalide content is what is unique to celery as a supplement/food, and the phthalide known as Sedanolide is the main bioactive in celery seed oil. There appears to be a large degree of variability as to the amount of volatiles in a source of celery seed oil
With trace (0.1% or less) amounts of cumene, nonane, α-Thujene, carveol, and Terpinen-4-ol.[7] The volatile oil is a large percentage of monoterpene hydrocarbons (46%) and a high source of phthalide compounds (42.3%).[5][8] The aerial parts of celery also contain phthalides, but are more variable (1-39.5% of total volatiles) than the seeds[12] with the vegetable stem containing 28.1% of total volatiles as the celery aromatic sedalonide.[2]
The aerial parts (water extract) contain irioids and coumarins, whereas the methanolic seed extract does not.[7]
2.
Neurology
2.1
Mechanisms
The ethanolic extract of celery leaves has been found to inhibit the MAO-A enzyme with an IC50 of 5μg/mL, being slightly less effective than fenugreek (4μg/mL).[4] The water extract was less effective, and all extracts of celery failed to significantly influence citalopram-binding to the serotonin receptor (suggesting no affinity).[4]
Ligustilide has been noted to activate TRPA1 channels (EC50 of 44μM for activation; IC50 for inhibition at 1,456μM) and aromatization of ligustilide to dehydroligustilide (occurs via exposure to the air or celery aging[14]) reversed this action (EC50 of 539μM yet an IC40 of 23μM);[9] it appears to act via its electrophilic properties via alkylation of cysteine residues on TRPA1 (antagonism from dehydroligustilide appears to be independent).[9]
3.
Interactions with Organ Systems
3.1
Liver
Celery oil (50µl/kg orally) for 6 weeks has shown protective effects in an animal model of DHEP toxicity (a phthalate alongside Bisphenol A[15][16] that targets PPARα and confers antiandrogenic properties given alongside the celery oil for 6 weeks) was able to attenuate the increase in relative liver weight and lipids as well as serum lipids and liver enzymes.[1]
The methanolic extract of the seeds has shown protective effects against thioacetamide and paracetamol (assessed by liver enzymes in serum and histological examination).[3]
3.2
Stomach
In an ulcer test, 300mg/kg of the methanolic and water extracts of celery seeds in a gastric ulcer test (induced by alcohol) was able to inhibit 91% and 95% of the ulceration, respectively; a similar potency to the active control omeprazole (94%).[7] Lower doses (100-200mg/kg) of water extracts of the aerial parts of celery were not significantly effective,[7] and this anti-ulcer effect against ethanol has been noted elsewhere.[6]
3.3
Testes
200mg/kg celery oil to rats also given the testicular toxic phthalate (DHEP) was able to exert protective effects when coingested.[17]
4.
Interactions with Cancer
4.1
Stomach
In human cancer BGC-823 cells, celery seed oil is able to induce apoptosis in a concentration and time dependent manner with 300µg/mL for 72 hours reaching near 70% apoptosis which was characterized by a decrease in cells in G0/G1 phase (58.38% to 38.72%) and increase in S phase (30.51 to 61.54%) and decreased expression of CDK2 and Cyclin A.[18]
In mammalian systems, one study using acute doses of sedanolide or other phthalides from celery noted reductions in tumor size and multiplicity (tumors induced by benzo(a)pyrene) which was thought to be secondary to inducing the antioxidant enzyme glutathione S-transferase (GST).[19]pyrene-induced forestomach cancer in mice by natural phthalides from celery seed oil|published=1993|authors=Zheng GQ, Kenney PM, Zhang J, Lam LK|journal=Nutr Cancer]
5.
Interactions with Oxidation
5.1
Genomoic oxidation
At least one study using Sedanolide from celery oil in vitro failed to find a significant protective effect of this molecule against H2O2 or tert-butyl hydroperoxide induced DNA damage.[20]
6.
Safety and Toxicity
Up to 5,000mg/kg of celery seed extract for 28 days in rats failed to result in any clinically significant side effects (a small rise in the liver weight of female rats, but was without pathological changes; a small increase in serum globulin and phosphorus in males that was small enough to be deemed not relevant).[21]
References
- ^El-Shinnawy NAThe therapeutic applications of celery oil seed extract on the plasticizer di(2-ethylhexyl) phthalate toxicityToxicol Ind Health.(2013 Feb 1)
- ^Volatile components of celery and celeriac
- ^Singh A, Handa SSHepatoprotective activity of Apium graveolens and Hygrophila auriculata against paracetamol and thioacetamide intoxication in ratsJ Ethnopharmacol.(1995 Dec 15)
- ^Jäger AK, Gauguin B, Andersen J, Adsersen A, Gudiksen LScreening of plants used in Danish folk medicine to treat depression and anxiety for affinity to the serotonin transporter and inhibition of MAO-AJ Ethnopharmacol.(2013 Feb 13)
- ^
- ^Antiulcerogenic activity of Apium graveolens seeds oils isolated by supercritical CO2
- ^Baananou S, Bouftira I, Mahmoud A, Boukef K, Marongiu B, Boughattas NAAntiulcerogenic and antibacterial activities of Apium graveolens essential oil and extractNat Prod Res.(2012 Aug 30)
- ^Volatile aroma constituents of celery
- ^Zhong J, Pollastro F, Prenen J, Zhu Z, Appendino G, Nilius BLigustilide: a novel TRPA1 modulatorPflugers Arch.(2011 Dec)
- ^Mei QB, Tao JY, Cui BAdvances in the pharmacological studies of radix Angelica sinensis (Oliv) Diels (Chinese Danggui)Chin Med J (Engl).(1991 Sep)
- ^Li XR, Liang YZ, Guo FQAnalysis of volatile oil in Rhizoma ligustici chuanxiong-Radix paeoniae rubra by gas chromatography-mass spectrometry and chemometric resolutionActa Pharmacol Sin.(2006 Apr)
- ^Marongiu B, Piras A, Porcedda S, Falconieri D, Maxia A, Frau MA, Gonçalves MJ, Cavaleiro C, Salgueiro LIsolation of the volatile fraction from Apium graveolens L. (Apiaceae) by supercritical carbon dioxide extraction and hydrodistillation: Chemical composition and antifungal activityNat Prod Res.(2012 Sep 14)
- ^Liu T, Liu FG, Xie HQ, Mu QPhytopathogenic fungal inhibitors from celery seedsNat Prod Commun.(2012 Jul)
- ^Schinkovitz A, Pro SM, Main M, Chen SN, Jaki BU, Lankin DC, Pauli GFDynamic nature of the ligustilide complexJ Nat Prod.(2008 Sep)
- ^Halden RUPlastics and health risksAnnu Rev Public Health.(2010)
- ^Olsén L, Lind L, Lind PMAssociations between circulating levels of bisphenol A and phthalate metabolites and coronary risk in the elderlyEcotoxicol Environ Saf.(2012 Jun)
- ^Madkour NKThe beneficial role of celery oil in lowering of di(2-ethylhexyl) phthalate-induced testicular damageToxicol Ind Health.(2012 Nov 16)
- ^Gao LL, Feng L, Yao ST, Jiao P, Qin SC, Zhang W, Zhang YB, Li FRMolecular mechanisms of celery seed extract induced apoptosis via s phase cell cycle arrest in the BGC-823 human stomach cancer cell lineAsian Pac J Cancer Prev.(2011)
- ^Chemoprevention of benzo[a
- ^Woods JA, Jewell C, O'Brien NMSedanolide, a natural phthalide from celery seed oil: effect on hydrogen peroxide and tert-butyl hydroperoxide-induced toxicity in HepG2 and CaCo-2 human cell linesIn Vitr Mol Toxicol.(2001 Fall)
- ^Powanda MC, Rainsford KDA toxicological investigation of a celery seed extract having anti-inflammatory activityInflammopharmacology.(2011 Aug)